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Experimental setup for a MSCC consisting of an automated live-cell imaging microscope as well as the respective pumping periphery and environmental control

Journal: STAR Protocols

Article Title: Protocol for microfluidic single-cell cultivation and live-cell imaging of Chinese hamster ovary suspension cell lines

doi: 10.1016/j.xpro.2024.103106

Figure Lengend Snippet: Experimental setup for a MSCC consisting of an automated live-cell imaging microscope as well as the respective pumping periphery and environmental control

Article Snippet: Camera for microscope (FX CMOS - Nikon DS-Qi2) , Nikon Europe B.V. , Cat#: MQA17599 (Europe).

Techniques: Live Cell Imaging, Microscopy, Control

Closing steps to guarantee issue-free MSCC (A) Inlet tubing is fixed to the automated microscope stage to prevent stress tension on the inlet. (B) The positioning of outlet tubing in the waste containers is checked to prevent flooding of the microscope.

Journal: STAR Protocols

Article Title: Protocol for microfluidic single-cell cultivation and live-cell imaging of Chinese hamster ovary suspension cell lines

doi: 10.1016/j.xpro.2024.103106

Figure Lengend Snippet: Closing steps to guarantee issue-free MSCC (A) Inlet tubing is fixed to the automated microscope stage to prevent stress tension on the inlet. (B) The positioning of outlet tubing in the waste containers is checked to prevent flooding of the microscope.

Article Snippet: Camera for microscope (FX CMOS - Nikon DS-Qi2) , Nikon Europe B.V. , Cat#: MQA17599 (Europe).

Techniques: Microscopy

Journal: STAR Protocols

Article Title: Protocol for microfluidic single-cell cultivation and live-cell imaging of Chinese hamster ovary suspension cell lines

doi: 10.1016/j.xpro.2024.103106

Figure Lengend Snippet:

Article Snippet: Camera for microscope (FX CMOS - Nikon DS-Qi2) , Nikon Europe B.V. , Cat#: MQA17599 (Europe).

Techniques: Recombinant, Cell Culture, Suspension, Software, Adhesive, Microscopy, Incubation, Transferring, Clinical Proteomics, Sterility